Ice core bacteria data from RVIB Nathaniel B. Palmerand ARSV Laurence M. Gould cruises NBP0104, LMG0106, NBP0204,and LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project; Sea Ice Microbes project) (NCEI Accession 0112364)

This dataset contains biological data collected on ARSV Laurence M. Gould and RVIB Nathaniel B. Palmer during cruises LMG0106, LMG0205, NBP0104, and NBP0204 in the South Atlantic Ocean, South Pacific Ocean, and Southern Ocean from 2001-07-28 to 2002-09-07. These data include bacterial abundance, bacterial carbon biomass, and chl_a_ugm. The instruments used to collect these data include Ice Corer. These data were collected by Dr Chris H. Fritsen of Desert Research Institute as part of the "GLOBEC: Sea Ice Microbial Communities (Sea Ice Microbes)" and "U.S. GLOBEC Southern Ocean (SOGLOBEC)" projects and "U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.

The following is the text of the dataset description provided by BCO-DMO:

Ice core bacteria data

Dataset Description:
Bacteria Abundance, Biomass and Chlorophyll a in Ice Cores

NOTES:
NBP0104: Cores labelled with "DNA" were collected for DNA analysis.

Contributor: Dr. Christian Fritsen University and Community College System of Nevada Desert Research Institute Div. of Earth and Ecosystem Sciences 2215 Raggio Parkway Reno, NV 89512

Office: 775/673-7487

BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination

Core Sampling techniques:

Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in:
Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. Polar Biol. , 6 :237-239.

Recommendations for reporting were used as outlined by:
Horner, R. et al. ,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. Polar Biol. 12 :417-427

Analytic Techniques:

Chla (mg m -3 ):

determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al ., 1984) ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting

Bacteria cell abundance (cells m -3 ) and biomass (mg C m -3 ):

LMG 0106

preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm 3 ; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV 0.86 ; Loferer-Krößbacher et al. , 1998). ice core samples corrected for FSW dilution

NBP 0104

preserved (0.5% glutaraldehyde) samples stained with SYBR® Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm 3 ), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV 0.59 ; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR® Gold-stained samples because both AO and SYBR® Gold stain bacteria cells similarly relative to DAPI (unpublished data). ice core samples corrected for FSW dilution

Loferer-Krößbacher, M., Klima, J., and R. Psenner. 1998. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Applied and Environmental Microbiology. 64:688-694.

Parsons,T.R., Maita, Y., and C.M. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press. Elmsford, New York.

Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series. 51, 201-213.

updated: April 20, 2006