Processing Steps |
- Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated.
- Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Amber 250 ml polyproplene bottles; Analyzing instrument: Alkalinity titrator; Cell type (open or closed): Open; CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography; CRM batch number: CRM 112 and CRM 98; Preservative correction: no correction.; Method reference: Used spectrometric method from Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp.; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: pH; Abbreviation: pH; pH scale: Total; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Amber 250 ml polyproplene bottles; Analyzing instrument: Varian Cary 100 Spectrometer; Temperature of pH measurement: 20 C; Detailed sampling and analyzing information: Varian Cary 100 Spectrometer, M-cresol purple Lot #1080-253; Uncertainty: Values were not corrected for the dye; Method reference: Used spectrometric method from Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp.; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: pCO2; Unit: uatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated.
- Parameter or Variable: Number of fish each tank was initially stocked with; Abbreviation: Initial number of fish; Unit: Number of individual fish; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Counting; Duration: 56 days; Detailed sampling and analyzing information: This variable describes the number of fish stocked into each tank at the start of the experiment; Replicate information: Fish were stocked into each tank once at the beginning of the experiment; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Number of live fish remaining in each tank at time of sampling; Abbreviation: Number of fish sampled; Unit: Number of individual fish; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Reponse variable; Measured or calculated: Measured; Sampling instrument: Counting; Duration: 56 days; Detailed sampling and analyzing information: each fish was individually counted while being weighed and measured; Replicate information: All live fish were sampled weekly for the duration of the experiment; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: mean total length of fish in each tank; Abbreviation: Fish length mean (mm); Unit: millimeters (mm); Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: measured; Sampling instrument: Metric fish measuring board; Analyzing instrument: Microsoft Excel was used to determine means; Duration: 56 days; Replicate information: All live fish were sampled weekly for the duration of the experiment; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: mean weight of the fish in each tank; Abbreviation: Fish weight (g); Unit: grams (g); Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: measured; Analyzing instrument: Mettler PM4600 balance; Duration: 56 days; Replicate information: All live fish were sampled weekly for the duration of the experiment; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Dissolved Oxygen as a percent of saturation; Abbreviation: D.O.% saturation; Unit: Percentage of saturation; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Placed YSI probe in each tank; Analyzing instrument: YSI 85, Microsoft Excel was used to deterine means; Duration: 56 days; Detailed sampling and analyzing information: YSI 85 was calibrated daily to an air temperature which matched the water temp being sampled +/- 1 degrees.; Replicate information: YSI readings were taken daily; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Dissolved Oxygen in millgrams per liter; Abbreviation: D.O. mg/L; Unit: Percentage of saturation; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Placed YSI probe in each tank; Analyzing instrument: YSI 85, Microsoft Excel was used to deterine means; Duration: 56 days; Detailed sampling and analyzing information: YSI 85 was calibrated daily to an air temperature which matched the water temp being sampled +/- 1 degrees.; Replicate information: YSI readings were taken daily; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Temperature in degrees Celcius; Abbreviation: Temp C; Unit: Degrees celsius; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Placed YSI probe in each tank; Analyzing instrument: YSI 85, Microsoft Excel was used to deterine means; Duration: 56 days; Detailed sampling and analyzing information: No calibration is required for this measurement; Replicate information: YSI readings were taken daily; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Salinity; Abbreviation: Salinity; Unit: Salinity units; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Placed YSI probe in each tank; Analyzing instrument: YSI 85, Microsoft Excel was used to deterine means; Duration: 56 days; Detailed sampling and analyzing information: No calibration is required for this measurement; Replicate information: YSI readings were taken daily; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: pH measurement tanken with handheld meter; Abbreviation: pH probe; Unit: millivolts; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Hach HQ11D; Duration: 56 days; Detailed sampling and analyzing information: Hach HQ11D was used with probe PHC28101. It was calibrated once a day prior to use with 4.01 and 10.01 buffer solutions; Replicate information: pH reading were taken daily; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Dissolved Silicate; Abbreviation: Silicate; Unit: umol/Kg; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: measured; Analyzing instrument: Quattro, Test range0-20umol/L, 10 mm flowcell with LED photometer at 820 nm, 60 samples/hr, standard deviation is 0.05 umol/L, detetion limit is 0.01 umol/L; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and ran immediately after collection; Method reference: Method NO Q-066-05 Rev. 3 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Dissolved Phosphate; Abbreviation: Phosphate; Unit: umol/Kg; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Analyzing instrument: Quattro, Test range 0-4 umol/L, 10 mm flowcell with LED photometer at 880 nm, 60 samples/hr, standard deviation is 0.01 umol/L, detetion limit is 0.004umol/L; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and ran immediately after collection; Method reference: Method NO Q-064-05 Rev. 3 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Area in square millimeters of the first otolith that was removed from each fish after the experiment was terminated; Abbreviation: Otolith #1 area mm2; Unit: Square millimeters; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Leica stereo microscope model EZ4D at 160X with camera attached; Analyzing instrument: Image J software; Duration: 56 days; Detailed sampling and analyzing information: Each otolith was extracted from the fish, cleaned, dried and photographed under the microscope. Next the photos were analyzed using Image J software to detmine the area. In some case we were unable to locate one or both otoliths or they broke during extraction. Those samples were not used.; Replicate information: Otoliths were removed once after the experiment was terminated; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: weight in grams of the first otolith that was removed from each fish after the experiment was terminated; Abbreviation: Otolith #1 wgt. (g); Unit: grams (g); Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Metler-Toledo balance model AB104-S/Fact balance; Duration: 56 days; Detailed sampling and analyzing information: Each otolith was extracted from the fish, wiped cleaned, dried and weighed on the balance. In some cases one or both otoliths or they broke during extraction.; Replicate information: Otoliths were removed once after the experiment was terminated; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: Area in square millimeters of the second otolith that was removed from each fish after the experiment was terminated; Abbreviation: Otolith #2 area mm2; Unit: Square millimeters; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Leica stereo microscope model EZ4D at 160X with camera attached; Analyzing instrument: Image J software; Duration: 56 days; Detailed sampling and analyzing information: Each otolith was extracted from the fish, cleaned, dried and photographed under the microscope. Next the photos were analyzed using Image J software to detmine the area. In some case we were unable to locate one or both otoliths or they broke during extraction. Those samples were not used.; Replicate information: Otoliths were removed once after the experiment was terminated; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service.
- Parameter or Variable: weight in grams of the second otolith that was removed from each fish after the experiment was terminated; Abbreviation: Otolith #2 wgt. (g); Unit: grams (g); Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Metler-Toledo balance model AB104-S/Fact balance; Duration: 56 days; Detailed sampling and analyzing information: Each otolith was extracted from the fish, wiped cleaned, dried and weighed on the balance. In some cases one or both otoliths were not found or they broke during extraction.; Replicate information: Otoliths were removed once after the experiment was terminated; Biological subject: Scup; Species ID: Stenotomus chrysops; Researcher name: Dean Perry; Researcher institution: National Marine Fisheries Service .
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