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Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) (NCEI Accession 0112521)

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This dataset contains biological data collected on ARSV Laurence M. Gould during cruises LMG0104 and LMG0106 in the South Pacific Ocean and Southern Ocean from 2001-04-29 to 2001-08-20. These data include bacterial abundance, bacterial carbon biomass, chl_a_ugm, and depth. The instruments used to collect these data include Niskin bottle. These data were collected by Dr Chris H. Fritsen of Desert Research Institute as part of the "GLOBEC: Sea Ice Microbial Communities (Sea Ice Microbes)" and "U.S. GLOBEC Southern Ocean (SOGLOBEC)" projects and "U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.

The following is the text of the dataset description provided by BCO-DMO:

Water column bacteria data

Dataset Description:
Bacteria Abundance, Biomass and Chlorophyll: Water Column Samples a

BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination

Core Sampling techniques:

Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in
Garrison and Buck (1986)

Recommendations for reporting were used as outlined by: Horner, R. et al.,(1992)

Analytic Techniques:

Chla (mg m -3 ):

determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al ., 1984) ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting

Bacteria cell abundance (cells m -3 ) and biomass (mg C m -3 ):

LMG 0106

preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm 3 ; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV 0.86 ; Loferer-Krossbacher et al. , 1998). ice core samples corrected for FSW dilution

NBP 0104

preserved (0.5% glutaraldehyde) samples stained with SYBR Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm 3 ), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV 0.59 ; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR Gold-stained samples because both AO and SYBR Gold stain bacteria cells similarly relative to DAPI (unpublished data). ice core samples corrected for FSW dilution

Data from LMG0106 (July-August, 2001) added in June 2002.

Updated: April 21, 2006
  • Cite as: Fritsen, Chris H. (2013). Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) (NCEI Accession 0112521). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0112521. Accessed [date].
gov.noaa.nodc:0112521
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2001-04-29 to 2001-08-20
Spatial Bounding Box Coordinates
West: -72.427
East: -67.049
South: -69.888
North: -65.965
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
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  • Fritsen, C. H. (2002) Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2002-11-15. https://doi.org/10.1575/1912/bco-dmo.2350.1
  • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2013-08-27
  • revision: 2023-03-30
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Acquisition Description:
# Bacteria data from Southern Ocean GLOBEC # C. Fritsen and F. Stewart # * BactAbun = bacterial abundance = cells m-3 # * BactBio = bacterial biomass = mg C m-3 = ug C l-1 # * chla = mg chla m-3 = ug chla l-1

In this accession, NCEI has archived multiple versions of these data. The latest (and best) version of these data has the largest version number.
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Fritsen, Chris H. (2013). Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) (NCEI Accession 0112521). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0112521. Accessed [date].
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Acknowledgments
  • Funding provided by NSF Antarctic Sciences (NSF ANT) Award Number: ANT-9910098 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=9910098
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Standard Parameters Global Change Master Directory (GCMD) Science Keywords Originator Parameter Names
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Platform keywords NODC PLATFORM NAMES THESAURUS BCO-DMO Platform Names Global Change Master Directory (GCMD) Platform Keywords ICES/SeaDataNet Ship Codes
Instrument keywords NODC INSTRUMENT TYPES THESAURUS BCO-DMO Standard Instruments Originator Instrument Names
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Place Names
Project keywords NODC PROJECT NAMES THESAURUS BCO-DMO Standard Programs BCO-DMO Standard Projects Global Change Master Directory (GCMD) Project Keywords Provider Cruise IDs Provider Funding Award Information
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Fritsen, Chris H. (2013). Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) (NCEI Accession 0112521). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0112521. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2013-08-27T13:46:09Z - NCEI Accession 0112521 v1.1 was published.
  • 2013-08-29T13:46:11Z - NCEI Accession 0112521 was revised and v2.2 was published.
    Rationale: Updates were received for this dataset. These updates were copied into the data/0-data/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession.
  • 2023-03-30T17:20:28Z - NCEI Accession 0112521 was revised and v3.3 was published.
    Rationale: Updates were received for this dataset. These updates were copied into the data/0-data/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession.
Output Datasets
Lineage information for: repository
Processing Steps
  • 2015-04-22T00:00:00 - NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
Acquisition Information (collection)
Instrument
  • bottle
Platform
  • R/V Laurence M. Gould
Last Modified: 2024-01-28T14:04:13Z
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