Skip to main content
Dataset Overview | National Centers for Environmental Information (NCEI)

Hypoxia and Hydrogen Sulfide (H2S) in Padilla Bay, WA from 2014-07-09 to 2014-09-30 (NCEI Accession 0156596)

browse graphicPreview graphic
Hypoxia has the potential to be a stressor to eelgrass as it can lead to tissue anoxia at night. These tissues then must undergo anaerobic metabolism, which is less energetically efficient and can produce toxic byproducts. Hypoxia may also work in synergy with other stressors, such as sediment pore-water sulfide. Hypoxia can facilitate the intrusion of sulfide, a known phytotoxin, into eelgrass tissues. This study examined the interaction between sulfide and hypoxia on the growth and photosynthetic efficiency of Zostera marina. Eelgrass shoots were collected from Padilla Bay, Washington and placed into seawater tanks in 18 oz. cups of sediment with a disk of agar at the bottom to simulate organic enrichment and to stimulate sulfide production. The growth rate and photosynthetic efficiency of the eelgrass shoots were monitored weekly for six weeks. After week three, the water columns of six of the tanks were reduced to hypoxic conditions (<2mg L-1). During week 6, the oxygen concentration was dropped further to near anoxic conditions. After week 6, eelgrass tissue samples were collected for measurement of total sulfur, carbon, and nitrogen. The results indicated that hypoxia had a significant negative effect on Z. marina shoots, which was evidenced by strong reductions in growth rates and photosynthetic efficiencies. These findings indicate that hypoxia in conjunction with sediment organic enrichment harms eelgrass health and enhances the intrusion of sulfide into plant tissues, over a wide range of pore-water sulfide concentrations.
  • Cite as: Ciesielski, Melissa; Shull, David; Yang, Sylvia (2016). Hypoxia and Hydrogen Sulfide (H2S) in Padilla Bay, WA from 2014-07-09 to 2014-09-30 (NCEI Accession 0156596). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0156596. Accessed [date].
gov.noaa.nodc:0156596
Full Text · XML
Download Data
  • HTTPS (download)
    Navigate directly to the URL for data access and direct download.
  • FTP (download)
    These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
Distribution Formats
  • Originator data format
Ordering Instructions Contact NCEI for other distribution options and instructions.
Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2014-07-09 to 2014-09-30
Spatial Bounding Box Coordinates
West: -122.49
East: -122.48
South: 48.49
North: 48.5
Spatial Coverage Map
General Documentation
Publication Dates
  • publication: 2016-10-18
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Submission Package ID: EENGYB
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Ciesielski, Melissa; Shull, David; Yang, Sylvia (2016). Hypoxia and Hydrogen Sulfide (H2S) in Padilla Bay, WA from 2014-07-09 to 2014-09-30 (NCEI Accession 0156596). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0156596. Accessed [date].
Cited Authors
Principal Investigators
Collaborators
Contributors
Resource Providers
Publishers
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science Keywords Provider Keywords
  • Total C:N:S
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords Provider Instruments
  • Apollo SciTech AS-C3 Dissolved Inorganic Carbon Analyzer
  • Elantech Flash EA 1112 elemental analyzer
  • ion selective electrode
  • ruler
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords Provider Place Names
  • Padilla Bay
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Ciesielski, Melissa; Shull, David; Yang, Sylvia (2016). Hypoxia and Hydrogen Sulfide (H2S) in Padilla Bay, WA from 2014-07-09 to 2014-09-30 (NCEI Accession 0156596). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0156596. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2016-10-18T14:59:10Z - NCEI Accession 0156596 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Data Type: PHOTOSYNTHETIC ACTIVE RADIATION (PAR) (measured); Units: micromole/second/m^2; Observation Type: laboratory analysis; Sampling Instrument: PAR light meter.
  • Data Type: Hydrogen Sulfide (H2S) (measured); Units: millimole; Observation Type: laboratory analysis; Sampling Instrument: ion selective electrode; Sampling and Analyzing Method: Pore-water sippers were used to obtain weekly sulfide concentrations in each sample. Sulfide present in pore-water was preserved in SAOB. Pore-water samples preserved in SAOB were measured using an ion selective electrode.; Data Quality Information: Calibration curves were created to ensure collected pore-water sulfide concentrations within samples were reasonable (0-10mM).
  • Data Type: growth rate (measured); Units: mm; Observation Type: other; Sampling Instrument: ruler; Sampling and Analyzing Method: Growth rates were measured weekly for each shoot using the pin-prick method. Shoots were marked along the sheath using a 23-gauge needle. Growth is calculated as new growth per day in mm and measured over 5 days. NAs are from shoots that had died.
  • Data Type: PHOTOSYNTHETIC CAPACITY (measured); Units: unitless; Observation Type: laboratory analysis; Sampling Instrument: fluorometer; Sampling and Analyzing Method: Photosynthetic efficiency was measured weekly using a PAM fluorometer. Plants were removed from the tanks and allowed to dark adapt for 30 minutes. Measurements were taken on the inner most, or youngest, leaf just above the sheath.; Data Quality Information: PAM was not measured for week 3. Otherwise, NAs indicate a shoot that had died.
  • Data Type: OXYGEN - DISSOLVED GAS [DISSOLVED OXYGEN] (measured); Units: milligram/L; Observation Type: laboratory analysis; Sampling Instrument: Oxygen sensor; Sampling and Analyzing Method: 6 out of 12 tanks were subjected to hypoxic conditions. During the first 3 weeks of the experiment all tanks were bubbled continuously with ambient atmospheric air to maintain dissolved oxygen levels at saturation. During the latter half of the experiment, 6 of the 12 tanks were treated with 500 ppm CO2-Nitrogen gas to reduce water column oxygen to <2 mg/L (hypoxic conditions). During the last week of the experiment, oxygen concentrations in hypoxic treatments were reduced to 0 mg/L to determine if eelgrass can survive anoxia.; Data Quality Information: Oxygen concentrations in hypoxic tanks were monitored daily using PreSens PSt3 oxygen sensors and HOBO U26 Dissolved Oxygen loggers. Oxygen concentrations in oxygen-saturated tanks were monitored using Clark-type micro electrode and picoammeter.
  • Data Type: Total C:N:S (measured); Units: NA; Observation Type: laboratory analysis; Sampling Instrument: Elantech Flash EA 1112 elemental analyzer; Sampling and Analyzing Method: All samples were stored frozen (-80C) and then were dried and ground using a mortar and pestle in preparation for elemental analysis, carbon, nitrogen and sulfur, using BBOT as the standard. Sample masses ranged from 3-10 mg. Samples, blanks, and standards were run with 10 mg of vanadium pentoxide to reduce tailing of the sulfur peak.
  • Data Type: DISSOLVED INORGANIC CARBON (DIC) (measured); Units: μmol kg-1; Observation Type: laboratory analysis; Sampling Instrument: Apollo SciTech AS-C3 Dissolved Inorganic Carbon Analyzer; Sampling and Analyzing Method: Water samples were collected and filtered through a 0.2 μm nylon filter. Samples were refrigerated at 2°C for one month before processing.
Acquisition Information (collection)
Instrument
  • fluorometer
  • oxygen sensor
  • PAR Sensor
Last Modified: 2024-02-26T15:21:43Z
For questions about the information on this page, please email: ncei.info@noaa.gov